HPLC-MS High Performance Liquid Chromatography Mass Spectometry

Function
* Separation of complex chemical mixtures using liquid chromatography and a variety of stationary phases
* Detection by Photo Diode Array for identification and quantification

* Identification of the compounds using a mass selective detecto
Advantages

* Extremely selective and sensitive
* Will separate non-volatile chemical compounds
Applications
* Analysis of high molecular weight phytochemicals and pharmaceuticals

PREP-HPLCPreparative High Performance Liquid Chromatography

Function
* Separation and collection of relatively large quantities of volatile and non-volatile chemical compounds
Advantages
* Greater selectivity in separation and collection of chemical compounds than traditional wet chemical techniques
Applications
* Preparation of pure compounds for chemical standards
* Isolation of components for identification and bioactivity

GC-MSGas Chromatography-Mass Spectrometry

Function
* Separation and identification of organic compounds using a gas-liquid system.
* Identification of the compounds using a mass selective detector
Advantages
* Identification of unknown compounds
* Extremely selective and sensitive
Applications
* essential oils
* pesticide residues
* odour chemicals
* pharmaceuticals

 

SFE - Super-Critical Fluid Extraction

Function
* Extraction of natural products using very high pressure CO2 as a solvent
Advantages
* Faster and more accurate than steam distillation
Applications
* Analysis of volatile compounds in:
Wood, Leaves and Powders