Scanning Electron Microscopy Unit
The scanning electron microscopy unit provides electron microscopy services to postgraduate students, staff members and commercial research projects/consultancies. The lab currently has a Leica Leo 440 Stereoscan Scanning Electron microscope capable of high resolution images (60,000X magnification). The Leica 440 is capable of secondary (SE), backscattered (BSE) imaging, and EDS X-Ray analysis.
- Electron microscopy imaging of biological and geological specimens.
- EDS X-Ray analysis of geological and soil samples.
- Specimen preparation - (oven drying, freeze drying, CPD drying)
- Training in Scanning Electron Microscopy.
Scanning Electron Microscope
Leo 440 Stereoscan Scanning Electron Microscope
- SE detector
- BSE detector (4 segment)
- Cathodoluminesence detector
- EDS X-Ray Analysis detector (Be window)
- Digital Imaging - 1024 x 768 pixels
- Large specimen chamber
- Resolution: 4.5nm at 30kV
- Accelerating voltage: 300V to 30kV
All chemicals and fixatives necessary for SEM sample preparation are available from the SEM/Microscopy unit. If they are not available they can be ordered. Dissecting microscopes, appropriate safety equipment and fume hoods are also available.
- Critical Point Drying: Polaron E3100 using Liquid CO2.
- Freeze-Drying: Christ Alpha 2-4 Ice condenser temperature-55oC, -85oC.
- Etching: - Polaron PT7150 Plazma barrel Etcher.
- Balzers Sputter Coater (Au, C and Pt coating available) evaporative coating attachment (Carbon).
- Ultramicrotome: Reichert OMU3 Ultramicrotome.
- Digital Imaging 1024 x 768 pixels from the Leo 440.
- Software available for processing - Adobe photoshop.
- Mitsubishi Video Copy Processor - P78E.
- Mitsubishi Color Video Copy Processor CP200B.
- KR-10X Pentax - B/W film 35mm format camera.
- Rodenstock large format - 70mm ("220") roll film.
- Olympus SZX16 Research Stereo Microscope (zoom range 7x - 115x). Phase contrast, polarizingand darkfield condenser. 35mm film camera, video and DP12 digital camera available (2048 x 1536 pixels).
- Olympus CH40 Research Compound Microscope (zoom range 40x 1000x). Phase contrast, polarizing and darkfield condenser. CX-RFL Reflected Light Fluorescence attachment. 35mm film camera, video and DP12 digital camera available (2048 x 1536 pixels).
- Olympus BHSM-NL-2 Metallurgical Polarizing Microscope (zoom range 50x 1000x). Incident/transmitted light and brightfield/darkfield. BH-2 C Mount for T.V. 35mm film camera and DP12 digital camera available (2048 x 1536 pixels).
- Micropublisher 3.3RTV - High resolution digital CCD colour camera - 2080 x 1542 pixels. High speed real time viewing.
- Olympus DP10 Digital camera - 2/3 inch primary color progressive CCD with 1.41 million pixels. (1284 x 1024 pixel quality).
- BH2-DA drawing attachment allows sketching of observed images through Olympus Compound Scopes. Magnification 20x 1000x.
- Macintosh computer with Olympus and Adobe photoshop software for processing of images with CD Burner available.
The following courses are available to postgraduate students (PhD, Hons) staff members (academic, general), and commercial research/consultancy groups. Contact us for relevant costs.
Introduction to the SEM
- This course covers Basic and Advanced SEM techniques as well as techniques in sample preparation.
Introduction to X-ray Analysis
- This course covers X-ray Anaylsis using energy dispersive (EDS) analysis.
- Please note: pre-requisite for this course is the Introduction to the SEM course.
Note: All new users will be trained on the SEM on a one-to-one basis by a technical officer.
t: 02 6620 3661
m: 0408 663 188
f: 02 6621 2669
Images were taken on the Leo 440 Scanning Electron Microscope by postgraduate students at SCU.
Updated: 04 March 2013